cell-based assay, bead-based assay, quanitfy protein production, quantify cellular responses

The CellReporter™ system produces accurate, quantitative data for a wide range of applications utilizing fluorescent cell- and bead-based assays. Intuitive workflows provide full flexibility to optimize and standardize assays from the first image acquisition step through to data analysis and reporting. Typical applications include bead-based assays for hybridoma screening, quantifying antibody production and secretion of cytokines or hormones and cell-based assays for cell cycle analysis, cytotoxicity studies or monitoring protein translocation.

Increase throughput without compromising data quality

  • View and analyze high resolution images at sub-cellular or individual bead level
  • Accurate object identification - differentiate between small objects within and around cells or beads

  • Compare morphology of individual cells using fluorescence and white light

Raw image of adherent CHO cells stained with propidium iodide-Texas Red filter set
Cell nuclei identified using propidium iodide
Untreated HeLa cells under white light
Untreated HeLa cells under fluorescence
HeLa cells stimulated by pro-apoptopic compounds under white light
HeLa cells stimulated by pro-apoptopic compounds under fluorescence

Optimize data analysis for each application

  • Analyze populations based on statistics obtained for each object
    • Locate and quantify fluorescence of each object
  • Multiple fluorescent channels offer flexibility in fluorophore choice, broadening application options

Improve data quality

Use scatter plots to review data, group and exclude undesired objects from analysis; e.g. fluorescent debris.

Example: Assessment of cell cycle via changes in nuclear DNA content

Heterogeneous cell population gated into sub-groups. Analysis within histogram is represented in images.

Report and export data

  • Visualize data as scatter plots, histograms, images, pie charts or heat maps
  • Normalize data to cell or bead number
View relative proportions of sub-groups per well
Export data for analysis

View the CellReporter workflow

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CellReporter™
Quantification of cellular responses
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Recent publications
Technical note: Analyzing Cell- and Bead-Based Assays
GEN Tech Notes: May 1, 2011 (Vol. 31, No. 9)
Paper that demonstrates how CellReporter offers a multipurpose solution for accurate and quantitative results for cell- and bead-based assays.
Read it now
Recorded webinars

Phenotypic Screening to Identify Cures for the Deadliest Poison

Dr. Tobin Dickerson, The Scripps Research Institute, San Diego, CA

Dr Dickerson discusses the development of a whole cell based assay for Botulinum neurotoxin (BoNT) inhibitors based on the new CellReporter™ multi-application platform. Listen now >

Accelerated high content screening and homogeneous quantification of soluble IgG levels

Dr Alasdair Robertson, Head of Biology R&D, Genetix Limited

Dr Robertson demonstrates how utilizing the CellReporter™ system's quantitative approach to assessing IgG levels can save time and simplify workflow compared to conventional methods such as FMAT®.

Listen now
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