cell-based assay, bead-based assay, quanitfy protein production, quantify cellular responses

The CellReporter™ system produces accurate, quantitative data for a wide range of applications utilizing fluorescent cell- and bead-based assays. Intuitive workflows provide full flexibility to optimize and standardize assays from the first image acquisition step through to data analysis and reporting. Typical applications include bead-based assays for hybridoma screening, quantifying antibody production and secretion of cytokines or hormones and cell-based assays for cell cycle analysis, cytotoxicity studies or monitoring protein translocation.

Increase throughput without compromising data quality

  • View and analyze high resolution images at sub-cellular or individual bead level
  • Accurate object identification - differentiate between small objects within and around cells or beads

  • Compare morphology of individual cells using fluorescence and white light

Raw image of adherent CHO cells stained with propidium iodide-Texas Red filter set
Cell nuclei identified using propidium iodide
Untreated HeLa cells under white light
Untreated HeLa cells under fluorescence
HeLa cells stimulated by pro-apoptopic compounds under white light
HeLa cells stimulated by pro-apoptopic compounds under fluorescence

Optimize data analysis for each application

  • Analyze populations based on statistics obtained for each object
    • Locate and quantify fluorescence of each object
  • Multiple fluorescent channels offer flexibility in fluorophore choice, broadening application options

Improve data quality

Use scatter plots to review data, group and exclude undesired objects from analysis; e.g. fluorescent debris.

Example: Assessment of cell cycle via changes in nuclear DNA content

Heterogeneous cell population gated into sub-groups. Analysis within histogram is represented in images.

Report and export data

  • Visualize data as scatter plots, histograms, images, pie charts or heat maps
  • Normalize data to cell or bead number
View relative proportions of sub-groups per well
Export data for analysis

View the CellReporter workflow

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CellReporter™
Quantification of cellular responses
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Recorded webinar

Phenotypic Screening to Identify Cures for the Deadliest Poison

Dr. Tobin Dickerson, The Scripps Research Institute, San Diego, CA

Dr Dickerson discusses the development of a whole cell based assay for Botulinum neurotoxin (BoNT) inhibitors based on the new CellReporter™ multi-application platform. Listen now >
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